Stéphane BAHRAOUI

En résumé

Entreprises

• Protein Metrics - Customer Success Scientist - Protein analysis should never be limited by instrument software!

  2018 - maintenant We believe that protein analysis should never be limited by instrument software! That’s why our software analyzes large, complicated data sets from almost any experiment and any vendor instrument. Our goal is to enable researchers and laboratories to attain clear scientific insights and develop new diagnostics and therapeutics. Our software is now helping labs in 18 of the top 20 biopharmaceutical companies.
  
  - Training & Support existing deployments, with a strong focus on EU customers,
  - Demo & Support the sales team in developing new accounts relationships worldwide
  - Develop direct relations with kOL at companies using or considering Protein Metrics' solutions
  - Delight to serve as a liaison between Sales, Customers and the Development Team
  
  We’re an optimistic and passionate group of engineers and scientists focused on building the perfect software for characterizing complicated biomolecules. We have world-class expertise in algorithm design, scientific visualization, informatics, proteomics, glycomics, and biopharma product characterization, and we collaborate with top academic laboratories to advance what is possible in life science research.
  
  We talk with our customers about their data and analyses every day, and their challenges and requirements motivate us to continually extend the capabilities of our software.

• HRMS Application Specialist Support, Southern Europe - WATERS - HRMS Application Specialist Support, Southern Europe - WATERS

  2015 - maintenant Remote Field High Resolution Application Specialist - Biopharma - Southern Europe
  
  • Provide and deliver in a timely-fashioned on-site customer training and support for High Resolution Mass Spectrometry (Q-TOFs & Ion Mobility) systems associated Applications.
  • Specialized in Biopharmaceuticals as ADC, mAbs & Glycans, Customer support in France and Southern Europe (Fr, Es, It, Pt) field. Excellent customer communication & organization.
  • Develop and maintain technical expertise on Waters LC & MS systems, software products. Manchester European Demo Lab and Consumables departments for testing new instruments and sample preparation kits. Suggest product ideas and product improvement.
  • Establish a high level of communication with local Paris and Eur Demo labs. Quick learner.
  • Pre-sales and post sales skills support & demo. Good presentation and strengthen customer relationships/collaboration. Demonstrate drive and enthusiasm in work.
  • Support Sales & Marketing departments for business goals, customer success and satisfaction.
  
  Tags: Application Specialist, Engineer, Biopharma, ADC, mAbs, High Resolution Mass Spectrometry, HRMS, , HDMS, Synapt G2-Si, Xevo Q-ToF, G2XS, Ion Mobility, IMS, ETD, UNIFI, Antibodies, Glycans, Proteins, Peptides, Market, Customer, Meetings, Travel, France, Europe.

• SGS M-Scan, Geneva - Mass Spectrometry Analyst - Biopharmaceuticals Services

  2011 - 2015 SGS M-Scan is a world leading group of chemical analysis laboratories specialized in advanced chemical and biochemical testing as well as high-end GLP/GMP contract analytical services, consultancy and training based on Mass Spectrometry and Chromatography.
  
  Analytics, Protein /peptides & glycans chemistry expertise.
  • Customer service oriented and work experienced under GMP & GLP conditions.
  • GlycoProtein, peptide & polymers characterization by ES-MS, LC-MS and LC-MS/MS (Q-TOF Micromass, UPLC - Xevo G2S HRMS, MALDI-TOF/TOF)
  • GC-MS: Method Development, identification and quantification of small molecules, Glycans analysis: Monosaccharide composition & Linkage (Clarus 680-600T Perkin Elmer)
  • Quantification of Peptides and small molecules (MRM: UPLC-MS/MS; Waters XEVO TQS / Quattro Premier XE / 1200 Agilent - 4000 Q-Trap Applied)).
  • SDS PAGE, IEF analyses. 2-D Gels: Implementation and method development.
  • Generate GMP, GLP reports and Performance Qualifications. Results interpretation and reporting. Method and Instruments troubleshooting and maintenance.
  • Supply & Chemicals logistic and management. Procurement (Oracle) Contracts negotiation, and suppliers selection and contact.
  • In-process improvement with Lean and 5S systems. Storage management and control.

• Novimmune SA - QC / Analytical Senior Manufacturing Assistant

  2008 - 2011 Novimmune is a Swiss biotech company focused on the discovery and development of antibody-based drugs for the targeted treatment of inflammatory diseases, immune-related disorders, and cancer.
  - Manage and perform Drug Product and Drug Substance Stability Studies, Pre-Formulation and Giving Set Studies according to documentation and quality standards.
  - Lab management (Timelines, stock of supplies, equipment, system users). Method development and qualification.
  - Management, Training and Supervision of lab technicians.
  - Analysis and characterization of monoclonal antibodies by: IEX, prot-A, SEC-HPLC, MALLS(Alliance 2690 / Waters, Wyatt), Particle size & counting system (Royco - Pharmspec, Malvern), SDS PAGE and IEF electrophoresis, densitometry (Alpha Imager), ImmunoAssay (ELISA, HCP, LAL) with UV or fluorescence.
  - Implementation and method development of BioAnalyzer 2100 Agilent for Bi-Specific Antibodies (CE-SDS chips, microfluidics),
  - Reviewing and adaptation of SOPs for the QC/analytical laboratory.
  - Perform and design Qualification and Technology Transfert for severals project. Coordination with preclinical and clinical teams.
  - Stability Study Storage Monitoring (Oceasoft).
  - QCE: Quality Correspondant Equipment (Bioprocessing Area).
  - QA documentation (Deviation, amendement and CAPA)

• CNRS - IBCP/ IFR 128 BioSciences Gerland - Lyon Sud - Mass Spectrometry Internship - Master degree

  2007 - 2007 - Mass Spectrometry development: Improvement of sensibility of phosphorylated peptides with MALDI-TOF ( bibliography, Method development and support).
  - Derivatization of phosphorylated peptide with matrix using BEMAD method (beta-elimination, Michael-addition Derivatization). IMAC purification.
  - MALDI-TOF: Analysis of derivated proteins and use of differents conditions to improve peaks. (MALDI-TOF Voyager DE Pro – Applied Biosystem)

• Covance CLS - Medical technologist - Special Chemistry / Toxicology (temp position)

  2007 - 2008 - Perform testing of Drugs analysis by gas chromatography/mass spectrometry (GC/MS) Agilent HP 6890 / 5973 according to SOP. Sample preparation, SPE and derivatization. TLC.
  - Perform laboratory testing, Ensure the validity and the reliability of results,
  - Optimize reagents management, Maintain equipments and instruments, Document work as needed, Comply with safety rules.
  - GMP & GCLP environnement.

• CEA, Institut de Biologie Structurale (IBS), Grenoble Internship - Mass Spectrometry Internship - Master degree

  2006 - 2006 Mass Spectrometry (MS) protein characterization and quality control. - Using instrument as MALDI-TOF (Voyager Applied), Q-TOF (Micromass), and Ion-Trap (Esquire 3000) with HPLC (Agilent 1100).
  - Determination of MW of different proteins and generation of reports. Tune instrument.
  - Strong learning and comprehensive of instruments in MS and HDX method.
  - Dr Eric FOREST, David LASCOUX and Dr David LEMAIRE

Formations

• Université Lyon 1 Claude Bernard DEA Génie des Procédés

  Villeurbanne 2006 - 2007 Master degree in Biochemistry

Réseau

• Elise DIXON

• Eric LAFAURIE

• Guillemette PONTINI

• Laurent SAINT-ANDRE

• Luis SIRERA GARCIA

• Mohammed MERZOUK

• Sabrina GARÇONNET

• Sébastien BURTIN

• Yassine IBALHAOUNE