Brice Thomassin

Management of the DNA Capillary Sequencing Services.
-Planning and realization of daily production
-Customers relationship, troubleshooting
-Data Analysis
-Maintenance and stock management

DNA automatic sequencing Applied Biosystems 3730 XL
- Planning of 1800 reactions per day
- Realization of sequencing reactions, 1000 per day
- Run protocol optimizations according to the matrix type
- Sequencing reaction purification
- Control of the run conditions
- Data transferring, result insertions in database
- Monitoring results up to customer service
- Maintenance and stock management

Basic knowledge of GS-FLX 454-Roche sequencer
- Realization of the different steps, DNA nebulisation, emulsion, breaking and run

PCR amplification:
- Realization. Purification .Quantification and analyse of the results

Extraction, purification and quantification of plasmid DNA

Samples receipt:
- Samples data capture, 700 per day, orders data capture 50 per day

Oligonucleotides automatic synthesis Expedite, 30 per day
- Controlling production, quality monitoring
- Synthesisers maintenance and stock management